Protein_Domain

Part:BBa_K4618502

Designed by: PO-YANG PENG   Group: iGEM23_CSMU-Taiwan   (2023-10-11)


bpuI + NG catcher

laccase from Bacillus pumillus codon optimised for E. coli and combine with NG catcher

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 825
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 825
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 825
    Illegal XhoI site found at 7
    Illegal XhoI site found at 164
    Illegal XhoI site found at 794
    Illegal XhoI site found at 1034
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 825
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 825
    Illegal AgeI site found at 816
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1132
    Illegal BsaI.rc site found at 1230
    Illegal BsaI.rc site found at 1656


[edit]
Categories
Parameters
None